Tecan Safire 2 Manual

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Tecan Safire 2 Manual Rating: 5,5/10 8685 votes
  1. Tecan Safire 2 User Manual
  2. Tecan Safire Ii Manual
Increase your productivity for advanced research and drug discovery with Spark, the first multimode microplate reader with integrated cooling.

Tecan Safire has been developed with the drug discovery process in mind. Due to its in-built flexibility, sensitivity and performance, Safire is the ideal instrument to bridge the gap between assay development and high throughput screening. Tecan Safire2™ Microplate Reader NOTE: The Tecan Safire 2 ™ Microplate Reader was tested for compatibility with Invitrogen's Predictor ™ hERG FP Assay (PV5365) using controls provided within the assay kit and the. Tecan Safire. Tecan Safire 2 Basic Microplate Reader W Options. Tecan Genesis. Tecan Genesis Instrument Software And Manual 615018, V. Tecan Cavro Mw Miniwash Diaphragm Pump And Smart Valve Sv 4 Port Liquid Handling. Tecan 30015374. An additional time, select “manual” for both the Gain and Z-Position, and enter the values determined by the instrument the first time the plate was read. If Read Mode can be changed, select “Top”. Set the “Time between move and read:” to. 608 • 443 • 2400 • • • • ® ®® •• •• Tecan Safire 2 FI. DownloadTecan safire 2 user manual. Get file 2008-07-16 19 13 172,144 -c-a-w c program files mozilla firefox components xpinstal. Tecan safire 2 user manual Get file - Tecan safire 2 user manual Mirror Link #1.

Spark® Multimode Microplate Reader


Average Rating: 4.4

4 out of 5
After sales service

  • Status:

    Reviewer
  • Member since: 2006

  • Organization: Novo Nordisk A/S



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5

'Very easy to use, very reliable results.'

Review date: 14 Feb 2019 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2018

  • Organization: Rexgenero



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Tecan Spark 10M together with Magellan are properly designed.
Rating: 4.3
'I think Tecan Spark 10M and Magellan are very user friendly in terms of software. The main advantage for me is the option to buy a GMP-accomplished version. It makes life much easier to researchers to track all the ELISA results for further audits and commercial approvals.'

Review date: 17 Jan 2019 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of Cyprus



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
In summary, this is a very high-quality instrument, with some basic limitations
Rating: 4.7
'The reader has specific qualities: very high sensitivity (quality optics, combination of monochromator and filters), combines modules like injector and incubator cassette for realt-time long assays in live cells, its easy-to-use, it has a friendly interface control software and is very reliable (very rarely it pops out messages of error etc). Tecan provides a very high-quality after-sales care, with online service (via chat) and quick email responses. They also provide customisation of their responses to particular assay-specific problems. One of the disadvantages of the software used in Tecan Spark 20M, is that it doesn't give you a calculation of the time-to-end of your assay screening after you've programmed the detection actions to be done. Another limitation is that the reader cannot do a BRET assay. Finally, the additional analytical software that can work with this reader (Magellan) is very very complicated and comprehensive when compared to the control-software, but the control-software has zero capacity for analysis (it just produces raw data in excel format).'

Review date: 23 Jul 2018 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2018

  • Organization: X-Chem Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Able to utilise instrument for a variety of assays e.g HTRF, AlphaScreen, FP
Rating: 5.0
'The instrument is easy to use. I especially love the z height function, it greatly improves sensitivity.'

Review date: 07 Feb 2018 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2016

  • Organization: ETH Zurich



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
'The system now after update of the software became an essential tool in the lab!'

Review date: 03 Aug 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: EYOWN TECHNOLOGIES S.L.



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
'The SPARK instrument has been the best reader for analyzing our samples, proceeded from being candidates for new drugs. TECAN support in Spain has been quietly involved in optimizing the software application to adapt it for our necessities. '

Review date: 27 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: CSK food enrichment b.v.



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.3
'The Tecan Spark 10M is very sensitive in its fluorescence range and works like a charm.'

Review date: 24 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2010

  • Organization: University of Leeds



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7
'Easy to use instrument for a large number of different applications'

Review date: 21 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Hoffmann-La Roche AG



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great instrument we use as an equivalent to our TECAN M1000pro
Rating: 4.0

  • Application Area:analysis of biologics by fluorescence, absorbance, etc.

'The machine produces good results as expected. Good value for money. '

Review date: 18 Apr 2017 Spark® Multimode Microplate Reader
Safire
  • Status:

    Reviewer
  • Member since: 2006

  • Organization: Novo Nordisk A/S



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Quantitative analysis of biomarkers

'Very easy to use. Especially the setup of new measurement technologies is very fast done as it is very intuitive. I do also like the possibility of running absorbance or fluorescence scans.'

Review date: 18 Apr 2017 Spark® Multimode Microplate Reader


  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University Hospital Dusseldorf, Germany



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Absolute must-have for our applications - would buy it again.
Rating: 4.7
'Works well. Highly reproducible results with complete Tecan-based drug screening pipeline. Immediate and thorough support if any problems occur.'

Review date: 18 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Aspen Pharma



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Ideal for fast reproducible absorbance measurements
Rating: 4.0

  • Application Area:Measuring absorbance in EIA determinations

'A device that is easy set up, has an intuitive interface, fast and robust.'

Review date: 14 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of North Texas HSC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:protein estimation, quantification of fluorescent & luminiscence intensity of the cells

'Its very easy to use. One can measure absorbance, luminescence, fluorescence and even RNA & DNA estimation in a single instrument.. really awesome.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of California San Diego



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Analyze luminescence and fluorescence

'The method editor is very convenient and easy to use for creating new protocols. The machine runs very quickly and efficiently. The injectors are easy to implement. If there's a will there's a way, the capabilities seem endless!'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Ucsd



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:protein binding and cell absorbance assay

'We got a very good training after purchase for using the equipment from our rep, Richard Cavicke. The equipment is running reliably. It's a good purchase.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: California Institute of Technology



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
I purchased a demo and have received good support from the sales/technical staff.
Rating: 4.3

  • Application Area:Luminescence, fluorescence, DNA quantification

'Software is easy to use. Follow-up by technical/sales support is good.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Tufts University



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:protein and DNA quantification assays

'Using the Spark is fast and easy, and the results are dependable - when it is working properly. We have had a few issues with our plate reader that has required service multiple times in the about 2 years we have had it. The problem is always with the same part, the cell counting module. It seems the first few times it was serviced, the issue was not fixed properly. Service requests have always been addressed quickly, although we have needed them more often than I feel we should have. Our instrument actually had to be serviced right away because the issue with the cell counting module was detected during set up.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of Oklahoma



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very good instrument. I will recommend this to other labs.
Rating: 5.0

  • Application Area:Monitor growth curves, fluorescent probe uptake into whole cells.

'Pros: The product is easy to use. User friendly software and also produce reliable and reproducible results. Cons: The Firmware updates must be done regularly. Errors keep cropping up. The design of taking out the injector out of the unit to prime and put it back and forth for injection is kind of difficult especially when injector is used a lot.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: CEROM



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:DNA/RNA quantification, FRET assays

'We purchased the Spark a few months ago mainly for DNA/RNA quantification and for FRET assays. There are so many options available when ordering the instrument that it can easily fulfil everyone's needs. The NanoQuant plate is so easy to use and only requires 2 uL to get quick and reliable results. The instrument can also handle any model of microplates. Our Spark is equipped with a monochromator for absorbance and filters for fluorescence assays. Setting up a new method is very simple, data manipulation can be done automatically by the software and exported in different file formats. I would recommend the Spark to any lab looking for a versatile plate reader.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Fox Chase Cancer Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Analysis of proteins from cell culture samples

'This product is extremely easy to use and reads plates fast. We primarily use it for absorbance and fluorescent measurements from media and lysate samples, but also live cells as well. Works wonderfully for all applications. As for maintenance and product support, the representatives are always available too and are very knowledgeable and helpful. Overall, great product from a great company.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Tufts University



  • Ease of use
    4 out of 5
    After sales service
    1 out of 5
    Value for money
    3 out of 5
Would recommend a more tested instrument. There seems to be some bugs with this model
Rating: 2.7

  • Application Area:Biological assays, DNA quantification

'When the instrument is working properly we have high quality results, however since purchasing the instrument we have had multiple issues with the cell counting module, which in turn has caused errors in instrument start up. Software is ease to use and mostly intuitive.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

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    Reviewer
  • Member since: 2017

  • Organization: Arcturus Therapeutics



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 4.0
'This machine is very easy to use and the result is reproducible'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of Nottingham



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7
'Great product. The Spark 10M Multimode Microplate Reader has added fast throughput capabilities to our spectroscopy laboratory. The easy to use interface and extensive capabilities make this a much used instrument. Not sure how we got by without it!'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: NIIOS (Netherlands Institute for Innovative Ocular Surgery)



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.7
'The device is quite intuitive in terms of ease of using; this concerns the software as well for data analysis. Hoover, the module for cell counting is not consistent in sample analysis. Multiple consecutive scans give fluctuating results; this means, 3 consecutive scans may provide 3 random cell countings.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of Warwick



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Essential piece of equipment for me, will expand its capability when I can afford it.
Rating: 4.7

  • Application Area:Microbiology (cell growth/reporter assays, metabolite quantification), ELISA.

'Easy to set up and use, very reliable and works almost full-time in my lab (including repeated 18-hour kinetic reads with eating and shaking). I would like to be able to export the graphic output form the dashboard as it seems there is no way to do this other than by taking a screen grab. I got a couple of quite for different machines and the Tecan seemed best value for money. I haven't given full marks for after sales care as I haven't needed to contact Tecan to solve any issues, so I can't comment on how good they are dealing with problems. But they have been in touch to check everything is working.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of Nottingham



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3
'Very easy to use and to set up protocols. Injector system is very helpful though I wish the tubing could be removed easily for better cleaning.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: AmatsiSEPS



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
This platereader is a very versatile and fast instrument
Rating: 4.3
'This platereader is a very versatile instrument and the main reason that we bought the instrument is for the ease, speed and the different applications we can use it for. This machine is of great value for our company, since we can adapt the settings to almost every application we have.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Newcastle University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:dynamic extracellular pH measurement

'The SPARK plate reader is easy to use and very intuitive. I have been able to set up a new method to measure real time changes in extracellular pH using fluorescent probes. I am able to perform kinetics analysis and with the temperature/CO2 control and humidity cassette, I can do long kinetics over the day. The z-scan function is very helpful for the type f experiments I do. We have had a few problems at the beginning but the engineers came over to have a look at it, sent it back to be fixed and provided us with a replacement demo machine in the meantime.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Glowee



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
'We already had a similar product, so nothing much to add. This machine fulfills what we had in mind. The TeCool option is quite interesting. Yet, we regret that it does not go less than 18 degrees celcius'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2016

  • Organization: ETH Zurich



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Simple to use and very effective but suffered of stability problems
Rating: 4.3

  • Application Area:Analysis of adherent cancer cell lines growth

'The system had several issues at the beginning and needed to be replaced a couple of times. Now the system is stable and it became a very precious tool for our research in the lab. Without the stability problem, which had a significant impact on our work I would have been completely satisfied with the machine. I still believe the software can be improved.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Norbitec GmbH



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Easy handling of the spark makes it fun to work with
Rating: 4.3

  • Application Area:analyzing total protein content and enzyme activity in cell culture samples

'There is a bit of work to be done to adapt the spark 10M in Terms of z-height and gain to achieve best results, but the after-sales-service was always there to give information on how to solve our problems. Right now we are establishing two methods for two different tests which will be very useful for us in the lab to gain a deeper insight into our cell culture Performance. The surface to set up a method is easily understood and very flexible in terms of adding or reducing the number of samples or blocks to be used in each method.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Uniklinik-freiburg



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:luciferase, proliferation assay, protein estimation

'Sophisticated and high quality instrument. It is easy to use with several applications possible. Reproducible results obtained.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: InSphero AG



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Analyze absorbance/luminescence/fluorescence based assay endpoint and cell counting

'After-sales care is excellent!! Received a comprehensive training on the magellan software! Spark is convenient, fast, sensitive and easy to use.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Biomillenia

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  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    4 out of 5

Tecan Safire 2 User Manual

Rating: 3.7
'Easy to use and good value, reproducible results. Instrument is upgradeable post-purchase, so additional functionality can be added as needed.'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2017

  • Organization: Uniklinik-Freiburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Rating: 4.3
'This machine is easy to use and intuitive even without any knowledege or introduction to the machine'

Review date: 13 Apr 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2009

  • Organization: Astrazeneca



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
'Since we have been using Tecan Safire 2 for many years, we chose the Spark 20M for assay and data consistency. An advantage is its absorbance OD window is quite a lot bigger than similar readers from other manufacturers. A disadvantage is the reading speed is quite slow.'

Review date: 18 Jan 2017 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2016

  • Organization: University of Salzburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent and innovative product. Highly recommended.
Rating: 5.0
'Highly recommended. New functions such as heater and stirred for the dispenser, full atmospheric control, humidity chamber. We were able to automatically record metabolic processes in live cells up to 72 hrs '

Review date: 24 Feb 2016 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2016

  • Organization: LAS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
'Best product - highly flexible to use for different application with reproducible results. High end instrument once you talk about kinetics based assay. '

Review date: 16 Feb 2016 Spark® Multimode Microplate Reader
  • Status:

    Expert Reviewer
  • Member since: 2015

  • Organization: Bharat Serums and Vaccines Ltd



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:For cell-based assays and DNA quantification

'Very useful machine, can do multiple applications, very fast and convenient to use'

Review date: 10 Feb 2016 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2010

  • Organization: University of Leeds



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very versatile instrument providing reliable results!
Rating: 5.0

  • Application Area:Analyze endpoint assays using absorbance and fluorescence

'Easy to use instrument for many different applications.'

Review date: 18 Dec 2015 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2015

  • Organization: SIRION Biotech GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
'Excellent cell counting algorithm suitable for a broad spectrum of cell types. Highly reliable cell detection when compared side by side with manual cell counting in a Neubauer chamber. Even resolution of small cell clusters does not pose a problem. Easy, very intuitive handling without complex adjustment of the instrument guarantees reliable and experimenter-independent results. The minimum number of cells required to obtain reliable results is as low as that needed for manual counting in a Neubauer chamber. In summary, among the well-established devices for automated cell counting which were all tested in our labs the Tecan Spark 10M possesses the best automated cell detection algorithm which in our opinion is currently available on the market.'

Review date: 12 May 2015 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2015

  • Organization: University of Salzburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Spectroscopic analysis of novel dyes, cell based assays, bacterial growth curves, cell counting..

'The new Tecan Spark 10M is the logical and consistent further development of the successful Infinite reader series. I appreciate the option to combine the high sensitivity of a filter with the flexibility of the monochromator measurement. As all relevant features for environmental control are now fully integrated into the reader and the software, I am convinced the Spark will become a very valuable tool for analysis of biological processes. The new cell counting function has proven rapid, precise and very helpful in my laboratory and has fully replaced the conventional counter. '

Review date: 31 Mar 2015 Spark® Multimode Microplate Reader
  • Status:

    Reviewer
  • Member since: 2015

  • Organization: Institute of Physiology, Paracelsus Medical University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Various cell-based microplate assays, ABS/FI/LUMI-based quantifications

'This instrument has straight-forward and intuitive handling and programming, as well as a great range of applications/measurement modes. The cell counting module achieves the step up from the microplate reader towards automated imaging. Similar to the previous software versions (iControl), the full range of functions are easy-to-use by the software's clear modular programming design. Additional features including gas and temperature control, lid handling, dual injection and, evaporation protection allows for flexible and versatile experimental design of cell-based applications.'

Review date: 23 Mar 2015 Spark® Multimode Microplate Reader

Increase your productivity for advanced research and drug discovery with Spark, the first multimode microplate reader with integrated cooling. This freely configurable system gives researchers access to new techniques and features designed to enhance and streamline biochemical and cell-based workflows.

Tecan Safire Ii Manual

The Spark multimode microplate reader provides tailor-made solutions to suit virtually any drug discovery or advanced life sciences research application. Developed with cell-based assays in mind, it offers a host of software and hardware features, including a unique Te-Cool™ cooling module that delivers total temperature control, even below ambient.

At the heart of the instrument are Spark’s unique Fusion Optics, which combine the flexibility of monochromators with the sensitivity of filters. Designed to improve sensitivity and increase throughput, this set-up allows on-the-fly measurements, increasing productivity without compromising on data quality. This option features the latest generation QuadX Monochromators™ and dichroic mirrors which, combined with variable bandwidth selection and full wavelength flexibility, provide exceptional measurement performance and speed. This is complemented by a patent-pending High Speed Monochromator, which allows complete absorbance scans – from 200 to 1,000 nm – in under five seconds; the speed of a diode array is combined with the precision and flexibility of a monochromator, offering wide-ranging and detailed spectra with unsurpassed wavelength accuracy for rapid DNA and protein quantification.

Reliable results are dependent on a stable temperature. For most readers, the specified minimum temperature inside the measurement chamber is defined as a few degrees above ambient. This can vary significantly depending on the time of year and location, affecting your results. Temperature gradients can also occur across a microplate with many readers, leading to poor precision and variability. Spark’s innovative Te-Cool cooling module provides complete temperature control between 18 and 42 °C allowing you to maintain your specified temperature for long periods of time, for accurate and reliable results.

The Te-Cool cooling module complements Spark’s range of advanced features – including precise regulation of environmental conditions, built-in cell counting and viability assessment, and automated imaging and confluence measurements – designed to simplify your protocols and enable long-term, walkaway experiments. Productivity and reproducibility are further improved by the addition of a patented Humidity Cassette to provide evaporation protection for any SLAS-format microplate, with automated lid handling to precisely balance gas exchange and minimize the risk of contamination. These new options – combined with the ability to read up to 1,536-well microplates – ensure there is a Spark configuration to increase the productivity of your lab, both today and in the future.

The Spark multimode microplate reader offers walkaway:

  • reagent dispensing
  • temperature control, including the Te-Cool cooling module
  • fluorescence
  • fluorescence resonance energy transfer (FRET)
  • time-resolved fluorescence (TRF)
  • fluorescence polarization (FP)
  • absorbance
  • DNA/protein quantification
  • luminescence (flash and glow)
  • luminescence scanning
  • multicolor luminescence
  • cell counting
  • live-cell imaging and in-well confluence assessment
  • cell incubation
Editorial Article
Cell-Based Assays: Trending Technology this Month
31 May 2019
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Tecan's Spark Reader Aiding Research Into Rare Skin Disease
04 Jul 2018
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Science on a Ship: Diverse Microbes of the Deep, Dark Oceans
08 Aug 2017
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Identify Biomarkers for Earlier Diagnosis of Pancreatic Cancer
02 Aug 2017
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Better Understanding of Scaffold Proteins and Their Therapeutic Potential
07 Apr 2017
Product News
Tecan Offers Cost-Effective, Walkaway Automation of Live Cell Assays
22 Feb 2017

Product Overview

Spark® Multimode Microplate Reader

Manufacturer Tecan Available Worldwide

4.4 / 5.0 43 reviews

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ManualJohannes Fels

Novo Nordisk A/S

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